Partenaires

Logo CNRS Logo UPMC


 
 
 

Rechercher

Sur ce site

Sur le Web du CNRS

 
 
 



Accueil du site > Productions scientifiques > Séminaires à PHENIX > 2012 > Séminaire 22.02.2012 - 13:30

Séminaire 22.02.2012 - 13:30

par Ali Abou-Hassan, Guillaume Mériguet - 2 janvier 2012

Sandra Ristori de l’Université de Florence donnera un séminaire dont le titre est :

Cationic micelles as nanovectors for siRNA delivery

Le séminaire aura lieu le mercredi 22/02/2012 de 13h30 à 14h30 au laboratoire PECSA (Bat. F, 7e étage, porte 754).

Résumé

RNA interference (RNAi) is an evolutionary conserved mechanism for controlling gene expression. The key elements in this process are a class of 19-25 bp double strand RNAs, collectively termed Small Interfering RNA (siRNA), which were discovered 13 years and nowadays are considered a major potential pathway in non viral gene therapy. However, to make RNAi a biomedical reality, efficient delivery agents must be chosen. A large number nanovectors have been proposed and characterized, leveraging on the research developed these latest decades for DNA delivery.

Here I describe the formation of complexes between a 21 bp siRNA, which blocks the expression of hERG1 potassium channel (involved in the pathogenesis of several human cancers) and two types of dimeric cationic surfactants. These are tetrametyammonium bromide geminis and a triazine-based surfactant.

Complementary techniques such as SANS, SAXS and DLS were used to investigate the structure and size of siRNA/micelle complexes, while Zeta Potential allowed to follow the variation of surface charge upon adding siRNA to micelles.

The analysis of static and time resolved scattering patterns shows that siRNA/micelle complexes are internally structured coacervates nucleating from the micelle solution upon progressive addition of siRNA.

The wealth of information obtained by extensive characterization is a valuable pre-requisite to guide transfection experiments.